Ayini amashubhu eqoqo legazi le-EDTA futhi Asetshenziswa Kanjani?

Ekuhlolweni kwezokwelapha kanye nokuxilongwa komtholampilo kanye nokwelashwa,Amashubhu okuqoqa igazi e-EDTA, njengezinto ezisetshenziswayo ezibalulekile zokuqoqwa kwegazi, zidlala indima ebalulekile ekuqinisekiseni ubuqotho bamasampuli kanye nokunemba kokuhlolwa. Kulesi sihloko, sizohlaziya ngokucophelela lo “mnakekeli ongabonakali” emkhakheni wezokwelapha kusukela ezicini zencazelo, ukuhlukaniswa kombala, umgomo we-anticoagulation, inhloso yokuhlola kanye nezinga lokusebenzisa.

YiniIshubhu lokuqoqa igazi le-EDTA?

Ishubhu yokuqoqa igazi le-EDTA iwuhlobo lweshubhu lokuqoqa igazi eline-Ethylene Diamine Tetraacetic Acid noma usawoti walo, osetshenziselwa kakhulu ukuqoqwa kwamasampula egazi nokwelashwa kwe-anticoagulant. I-EDTA ingavimba ukugeleza kwe-coagulation ngokuchela ama-ion e-calcium egazini, ukuze kugcinwe igazi lisesimweni soketshezi isikhathi eside, futhi inikeze amasampula azinzile okuhlolwa komjikelezo wegazi kanye nebhayoloji yamangqamuzana. Ihlinzeka ngamasampula azinzile wendlela yegazi, ibhayoloji yamangqamuzana nokunye ukuhlola.

Njengengxenye ebalulekile yeizinto ezisetshenziswa ezokwelapha, Amashubhu okuqoqwa kwegazi e-EDTA adinga ukuthobela indinganiso kazwelonke “yeziqukathi zokuqoqwa kwesampula yegazi le-venous ukusetshenziswa kanye” (isb. GB/T 19489-2008) ukuze kuqinisekiswe ukusebenza kobunyumba, okungeyona i-pyrogenic nokungeyona i-cytotoxicity.

Imibala ehlukene yamashubhu okuqoqa igazi e-EDTA

Ngokwezindinganiso ezivamile zamazwe ngamazwe (njengeziqondiso ze-CLSI H3-A6), amashubhu okuqoqa igazi e-EDTA ngokuvamile ambozwa ngokunsomi (EDTA-K2/K3) noma okuluhlaza okwesibhakabhaka (i-sodium citrate exutshwe ne-EDTA) ukuze ahlukanise ukusetshenziswa:

Qaphela: Amanye amabhrendi angase afakwe ikhodi ngeminye imibala, hlola imiyalelo ngaphambi kokuyisebenzisa.

Indlela ye-Anticoagulation yamashubhu okuqoqa igazi e-EDTA

I-EDTA ngeqembu layo le-molecular carboxyl (-COOH) nama-calcium ions egazini (Ca²⁺) kuhlanganiswe ukwenza i-chelate ezinzile, ngaleyo ndlela kuvinjwe ukusebenza kwe-plasminogen, ivimbe inqubo yokuhlangana kwe-fibrinogen ibe yi-fibrin. Le anticoagulation inezici ezilandelayo:

1. Ukuqala okusheshayo kwesenzo: i-anticoagulation ingaqedwa phakathi kwemizuzu engu-1-2 ngemva kokuqoqwa kwegazi;

2. ukuzinza okuphezulu: amasampula angagcinwa isikhathi esingaphezu kwamahora angu-48 (isiqandisi singanwetshwa sibe ngamahora angu-72);

3. Uhlu olubanzi lwesicelo: lufanele ukuhlolwa okuningi kwe-hematology, kodwa hhayi ukuhlolwa kokusebenza kwe-coagulation noma i-platelet (amashubhu e-sodium citrate ayadingeka).

Izinto zokuhlola eziyinhloko zeshubhu lokuqoqa igazi le-EDTA

1. ukuhlaziywa kwegazi okujwayelekile: inani lamangqamuzana amhlophe egazi, imingcele yamangqamuzana abomvu egazi, ukugcwala kwe-hemoglobin, njll;

2. ukuhlonza iqembu legazi nokuqhathanisa: Iqembu legazi le-ABO, ukutholwa kwe-Rh factor;

3. ukuxilongwa kwamangqamuzana: ukuhlolwa kofuzo, ukunqunywa komthamo wegciwane egazini (isb. I-HIV, i-HBV);

4. i-glycated hemoglobin (HbA1c): ukuqapha ushukela wegazi wesikhathi eside ku-mellitus yesifo sikashukela;

5. ukuhlolwa kwe-parasite yegazi: I-Plasmodium, ukutholwa kwe-microfilariae.

Ukusetshenziswa kwezinkambiso nezinyathelo zokuphepha

1. Inqubo yokuqoqa:

Ngemva kokubulala amagciwane esikhumbeni, sebenza ngokwezinga lokuqoqwa kwegazi le-venous;

Ngokushesha ngemva kokuqoqwa, guqula ishubhu lokuqoqa igazi izikhathi ezingu-5-8 ukuze uqinisekise ukuthi i-anticoagulant ixubene negazi ngokugcwele;

Gwema ukuzamazama okunamandla (ukuvimbela i-hemolysis).

2. Isitoreji nezokuthutha:

Gcina ekamelweni lokushisa (15-25 ° C), gwema ukushisa noma ukuqandisa;

Beka mpo ngesikhathi sokuthutha ukuze uvimbele ukuxegiswa kwekepisi yeshubhu.

3. Izimo ze-contraindication:

Amashubhu e-sodium citrate ayadingeka ku-Coagulation IV (PT, APTT, njll.);

Ukuhlolwa kokusebenza kweplatelet kudinga ishubhu ye-sodium citrate.

Indlela yokukhetha izinga eliphezuluIshubhu lokuqoqa igazi le-EDTA?

1. Ukufaneleka kanye nesitifiketi: khetha imikhiqizo ephumelele i-ISO13485 nesitifiketi se-CE. 2;

2. Ukuphepha kwezinto ezibonakalayo: umzimba weshubhu kufanele ube obala futhi ungabi nensalela ye-plasticizer;

3. Umthamo onembile: inani le-anticoagulant elengeziwe kufanele lihambisane nezinga likazwelonke (isb. Ukugxila kwe-EDTA-K2 kwe-1.8±0.15mg/mL);

4. Isithunzi somkhiqizo: Okuhamba phambili kunikezwa imikhiqizo eyaziwayo emkhakheni wezinto ezisetshenziswayo zezokwelapha ukuze kuqinisekiswe ukuzinza kweqoqo.

Isiphetho

Njengelungu elibalulekile leidivayisi yokuqoqa igazi, amashubhu okuqoqa igazi e-EDTA anomthelela oqondile ekunembeni kwemiphumela yokuhlolwa ngokwezakhiwo zawo ze-anticoagulant. Ngokumisa ukusetshenziswa kwamashubhu okuqoqa igazi anemibala ehlukene futhi uwahlanganise nezinqubo eziqinile zokuqoqa, kunganikeza isisekelo esithembekile sokuxilongwa emtholampilo. Esikhathini esizayo, ngokuthuthukiswa komuthi onembile, amashubhu okuqoqa igazi e-EDTA azodlala indima ebaluleke kakhulu ekuhlaziyeni igazi, ukulandelana kwezakhi zofuzo neminye imikhakha, futhi aqhubeke nokuvikela impilo yomuntu.