Ayini Amapayipi Okuqoqwa Kwegazi e-EDTA Futhi Asetshenziswa Kanjani?

Ekuhlolweni kwezokwelapha kanye nokuxilongwa kanye nokwelashwa kwezokwelapha,Amapayipi okuqoqwa kwegazi e-EDTA, njengezinto ezibalulekile ezisetshenziswayo zokuqoqwa kwegazi, zidlala indima ebalulekile ekuqinisekiseni ubuqotho bamasampula kanye nokunemba kokuhlolwa. Kulesi sihloko, sizohlaziya ngokuphelele lo "mqaphi ongabonakali" emkhakheni wezokwelapha kusukela ezicini zencazelo, ukuhlukaniswa kombala, isimiso sokuvimbela ukujiya kwegazi, inhloso yokuhlola kanye nezinga lokusetshenziswa.

KuyiniIthubhu lokuqoqwa kwegazi le-EDTA?

Ithubhu lokuqoqwa kwegazi le-EDTA uhlobo lwethubhu lokuqoqwa kwegazi elinomshini wokuhlanza eliqukethe i-Ethylene Diamine Tetraacetic Acid noma usawoti walo, elisetshenziswa kakhulu ekuqoqweni kwamasampula egazi kanye nokwelashwa kwe-anticoagulant. I-EDTA ingavimba ukusabela kwe-coagulation cascade ngokuchela ama-ion e-calcium egazini, ukuze igazi lihlale lisesimweni soketshezi isikhathi eside, futhi linikeze amasampula azinzile okuhlolwa kwesimiso segazi kanye ne-molecular biology. Inikeza amasampula azinzile okuhlolwa kwegazi, i-molecular biology kanye nezinye izivivinyo.

Njengengxenye ebalulekile yeizinto ezisetshenziswayo zezokwelapha, Amashubhu okuqoqwa kwegazi e-EDTA kudingeka ahambisane nendinganiso kazwelonke ethi “Izitsha zokuqoqwa kwesampula yegazi emithanjeni ezisetshenziswa kanye” (isib. GB/T 19489-2008) ukuqinisekisa ukusebenza kokungasebenzi kahle, okungena-pyrogenic kanye nokungabi na-cytotoxicity.

Imibala ehlukene yamapayipi okuqoqwa kwegazi e-EDTA

Ngokwezindinganiso ezivamile zomhlaba wonke (njengeziqondiso ze-CLSI H3-A6), amashubhu okuqoqwa kwegazi e-EDTA avame ukumbozwa ngombala onsomi (EDTA-K2/K3) noma oluhlaza okwesibhakabhaka (i-sodium citrate exutshwe ne-EDTA) ukuze kuhlukaniswe ukusetshenziswa:

Qaphela: Amanye amabhrendi angase afakwe ikhodi ngemibala ehlukile, hlola imiyalelo ngaphambi kokusebenzisa.

Indlela yokuvimbela ukujiya kwegazi yamapayipi okuqoqwa kwegazi e-EDTA

I-EDTA ngeqembu layo le-carboxyl yama-molecule (-COOH) kanye nama-ion e-calcium egazini (Ca²⁺) ahlangene ukwakha i-chelate ezinzile, ngaleyo ndlela evimbela ukusebenza kwe-plasminogen, evimba inqubo yokujiya kwe-fibrinogen ibe yi-fibrin. Lokhu kuvimbela ukujiya kunezici ezilandelayo:

1. ukuqala okusheshayo kokusebenza: ukuvimbela ukujiya kwegazi kungaqedwa zingakapheli imizuzu eyi-1-2 ngemva kokuqoqwa kwegazi;

2. Ukuqina okuphezulu: amasampula angagcinwa amahora angaphezu kwama-48 (efrijini anganwetshwa kuze kube amahora angama-72);

3. Uhla olubanzi lokusetshenziswa: lufanelekela ukuhlolwa okuningi kwe-hematology, kodwa hhayi ukuhlolwa kokuqina noma ukusebenza kwama-platelet (kudingeka amashubhu e-sodium citrate).

Izinto zokuhlola eziyinhloko zethubhu lokuqoqwa kwegazi le-EDTA

1. ukuhlaziywa kwegazi okuvamile: inani lamaseli amhlophe egazi, imingcele yamaseli abomvu egazi, ukuhlushwa kwe-hemoglobin, njll.;

2. ukuhlonza nokufanisa iqembu legazi: iqembu legazi le-ABO, ukutholwa kwe-Rh factor;

3. ukuxilongwa kwama-molecule: ukuhlolwa kofuzo, ukunqunywa komthamo wegciwane (isib. i-HIV, i-HBV);

4. i-hemoglobin ene-glycated (HbA1c): ukuqapha ushukela wegazi isikhathi eside kwesifo sikashukela;

5. ukuhlolwa kwezinambuzane egazini: I-Plasmodium, ukutholwa kwe-microfilariae.

Ukusetshenziswa kwemithetho kanye nezinyathelo zokuphepha

1. Inqubo yokuqoqwa:

Ngemva kokuhlanza isikhumba nge-disinfecting, sebenzisa ngokwezinga lokuqoqwa kwegazi emithanjeni;

Ngokushesha ngemva kokuqoqwa kwegazi, guqula ithubhu lokuqoqwa kwegazi izikhathi ezingu-5-8 ukuqinisekisa ukuthi i-anticoagulant ixubene ngokuphelele negazi;

Gwema ukuthuthumela ngamandla (ukuvimbela i-hemolysis).

2. Ukugcinwa kanye nokuthuthwa:

Gcina ekamelweni lokushisa (15-25°C), gwema ukushisa noma ukubanda;

Beka uqonde phezulu ngesikhathi sokuthuthwa ukuze uvimbele ukukhululeka kwesivalo sethubhu.

3. izimo zokuphikisana:

Amashubhu e-sodium citrate ayadingeka ukuze kuhlanganiswe i-Coagulation IV (PT, APTT, njll.);

Ukuhlolwa komsebenzi weplatelet kudinga ishubhu le-sodium citrate.

Indlela yokukhetha ikhwalithi ephezuluIthubhu lokuqoqwa kwegazi le-EDTA?

1. Iziqu kanye nesitifiketi: khetha imikhiqizo ephumelele isitifiketi se-ISO13485 kanye ne-CE. 2;

2. Ukuphepha kwezinto: umzimba wepayipi kufanele ubonakale futhi ungabi nama-plasticizer residue;

3. Ukulinganisa okunembile: inani le-anticoagulant elingeziwe kufanele lihambisane nezinga likazwelonke (isb. ukuhlushwa kwe-EDTA-K2 okungu-1.8±0.15mg/mL);

4. Idumela lomkhiqizo: Kubekwa phambili imikhiqizo eyaziwayo emkhakheni wezinto ezisetshenziswayo zezokwelapha ukuqinisekisa ukuqina kweqembu.

Isiphetho

Njengelungu elibalulekile le-idivayisi yokuqoqa igazi, amashubhu okuqoqwa kwegazi e-EDTA anomthelela oqondile ekunembeni kwemiphumela yokuhlolwa ngokwezakhiwo zawo zokulwa nokuqhekeka kwegazi. Ngokubeka amazinga ajwayelekile okusetshenziswa kwamashubhu okuqoqwa kwegazi anemibala ehlukene nokuwahlanganisa nezinqubo eziqinile zokuqoqwa, kunganikeza isisekelo esithembekile sokuxilongwa kwezokwelapha. Esikhathini esizayo, ngokuthuthukiswa kwemithi enembile, amashubhu okuqoqwa kwegazi e-EDTA azodlala indima ebaluleke kakhulu ekuhlaziyweni kwegazi, ukulandelana kwezakhi zofuzo kanye neminye imikhakha, futhi aqhubeke nokuvikela impilo yabantu.