Ukuhlola Okulahlwayo Kwezokwelapha Ishubhu Leqoqo Legazi Le-Lithium Heparin Anticoagulant eluhlaza
Incazelo
Ishubhu leqoqo legazi elincane linokwakheka komuntu kanye nekepisi elivaliwe lokuphepha, ishubhu lingavimba ngempumelelo ukuvuza kwegazi.Ngenxa yokwakheka kwayo okunamazinyo amaningi kanye nokuma ngakubili, ilungele ukuthuthwa okuphephile nokusebenza okulula, ngaphandle kokuchaphazeka kwegazi.
Ikhodi yombala yekepisi yokuphepha ihambisana ne-International Standard, Kulula ukuhlonza.
Idizayini ehlakaniphile yonqenqema lomlomo weshubhu kulula kubasebenzisi abafaka igazi ngefosholo eshubhuni.Elula, esheshayo futhi enembile, umthamo wegazi ungafundwa kalula ngomugqa ocacile wokuthweswa iziqu.
Ukwelashwa okukhethekile ngaphakathi kweshubhu, kubushelelezi ngaphandle kokunamathela kwegazi.
Ingakwazi ukwenza ibhakhodi ngendlela oyifisayo, futhi ifake inzalo kushubhu ngemisebe ye-gamma ngokwezidingo zeklayenti ukuze kufinyelelwe ukuhlolwa kwe-asepsis.
Ukwahlukaniswa Komkhiqizo
1. I-Plain (akukho okungeziwe, i-serum) tube (Ikepisi elibomvu);
2. I-clot activator (Pro-coagulation) tube(Ikepisi elibomvu);
3. Ishubhu ye-gel clot activator (SST)(Ikepisi eliphuzi);
4. Glucose (sodium fluoride, oxalate) tube (Grey cap);
5. Ithubhu ye-Sodium Citrate (1: 9) (I-cap eluhlaza okwesibhakabhaka);
6. Amashubhu e-Sodium (Lithium)Heparin (Ikepisi eliluhlaza);
7. Ishubhu le-EDTA K2 (K3, Na2)(Ikepisi eliPurple);
8. Ishubhu le-ESR (1:4) (Ikepisi elimnyama).
Imininingwane Yomkhiqizo
1. I-Gel & Clot Activator Tube
Ishubhu ye-gel neclot activator isetshenziselwa i-serum biochemistry, ukuhlolwa kwe-immunology kanye nokuhlolwa kwezidakamizwa, njll. Lapho ngokufanayo kufafaza i-coagulant ebusweni ngaphakathi kweshubhu, okuzonciphisa kakhulu isikhathi sokujika.
Njengoba ijeli yokuhlukanisa engenisiwe evela eJapane iyinto ehlanzekile, izinzile kakhulu endaweni ye-physicochemical, ingamelana kahle nezinga lokushisa eliphezulu ukuze ijeli igcine isimo esizinzile ngesikhathi sokugcina kanye nenqubo yokuthutha.
Ijeli izoqina ngemva kokufakwa phakathi futhi ihlukanise ngokuphelele i-serum kumaseli e-fibrin njengesithiyo, esivimbela ngempumelelo ukushintshana kwezinto phakathi kwe-serum yegazi namaseli.Ukusebenza kahle kokuqoqwa kwe-serum kuyathuthukiswa futhi kuzotholakala i-serum yekhwalithi ephezulu, ngaleyo ndlela iza emiphumeleni yokuhlola eyiqiniso.
Gcina i-serum izinzile amahora angaphezu kwama-48, alukho ushintsho olusobala oluzokwenzeka ezicini zayo zamakhemikhali ezinto eziphilayo kanye nokwakheka kwamakhemikhali, khona-ke ishubhu lingasetshenziswa ngokuqondile ekuhlaziyeni amasampula.
- Isikhathi sokuhoxiswa kwehlule eliphelele: 20-25min
- Isivinini se-Centrifugation: 3500-4000r / m
Isikhathi se-Centrifugation: 5min
- Izinga lokushisa elinconyiwe lesitoreji: 4-25ºC
2.I-Clot Activator Tube
I-clot activator tube isetshenziswa ekuqoqweni kwegazi le-biochemistry kanye ne-immunology ekuhlolweni kwezokwelapha.Ifanele ububanzi obubanzi bokushisa okusebenzayo.Ngokwelashwa okukhethekile, indawo engaphakathi ye-tube ishelela kakhulu lapho i-coagulant yekhwalithi ephezulu ifutha ngokufana.Isampula yegazi izothintana ngokuphelele ne-coagulant futhi ihlule phakathi kwe-5-8min.Ngakho-ke, i-serum yekhwalithi ephezulu itholakala ngokuhamba kwesikhathi nge-centrifugation, ngaphandle kokuqhekeka kwe-blood corpuscle, i-hemolysis, ukuhlukaniswa kweprotheni ye-fibrin, njll.
Ngakho-ke i-serum ingahlangabezana nezidingo zomtholampilo osheshayo kanye nokuhlolwa kwe-serum ephuthumayo.
- Isikhathi sokuhoxiswa kwehlule eliphelele: 20-25min
- Isivinini se-Centrifugation: 3500-4000r / m
Isikhathi se-Centrifugation: 5min
- Izinga lokushisa elinconyiwe lesitoreji: 4-25ºC
3.EDTA Tube
Ishubhu le-EDTA lisetshenziswa kakhulu ku-clinical haematology, ukufanisa okuphambene, ukuqoqa igazi kanye nezinhlobo ezihlukahlukene zamathuluzi okuhlola amangqamuzana egazi.
Inikeza ukuvikelwa okuphelele kwengqamuzana legazi, ikakhulukazi ukuvikela i-platelet yegazi, ukuze ikwazi ukumisa ngempumelelo ukuqoqwa kwe-platelet yegazi futhi yenze ifomu nomthamo wengqamuzana legazi lingathonywa phakathi nesikhathi eside.
Izingubo ezinhle kakhulu ezinekhono lomzuzu omkhulu zingafafaza izithasiselo ngokufanayo endaweni engaphakathi yeshubhu, ngaleyo ndlela isifanekiso segazi singahlangana ngokuphelele nesithasiselo.I-EDTA anticoagulant plasma isetshenziselwa ukuhlolwa kwezinto eziphilayo ze-pathogenic microorganism, i-parasite kanye ne-molecule yebhaktheriya, njll.
4.DNA Tube
1.Ishubhu legazi le-RNA/DNA eligcwaliswe ngaphambili ngesiphehlisi esikhethekile ukuze kuvikelwe ngokushesha i-RNA/DNA yesampula ukuthi ingacekelwa phansi.
2.Izibonelo zegazi zingagcinwa izinsuku ezi-3 ku-18-25°c, zigcinwe izinsuku ezinhlanu ku-2-8°c, zihlale zizinzile okungenani izinyanga ezingu-50 ku-20°c kuya ku-70°c.
3.Kulula ukuyisebenzisa, ukuguqula kuphela ishubhu legazi le-RNA/DNA ngezikhathi eziyisi-8 ngemva kokuqoqwa kungaxutshwa igazi kakhulu.
4.Faka egazini elisha labantu nezilwane ezincelisayo, alifanele igazi eligugiswe isikhathi kanye negazi elijiyayo kanye nelenkukhu nezinye izilwane.
5.Ukuqoqwa okujwayelekile, ukugcinwa kanye nokuthuthwa kwesampula yegazi lonke i-RNA/DNA
6.Udonga lwangaphakathi lweshubhu luwukucubungula okukhethekile ngaphandle kwe-RNase, i-DNase, qinisekisa ukubaluleka kwezibonelo zokutholwa kwe-nucleic acid
7.Ivumela ukukhishwa kwenqwaba nokushesha kwezibonelo, kuthuthukisa ukusebenza kahle kwelabhorethri
5.ESR Tube
I-Ø13×75mm ESR Tube isetshenziswa ngokukhethekile ekuqoqweni kwegazi kanye ne-anticoagulation ye-Automated Erythrocyte Sedimentation Rate Analyzers test sedimentation rate nesilinganiso sokuxuba sengxenye engu-1 ye-sodium citrate ezingxenyeni ezi-4 zegazi, ngendlela yase-Westergren.
6.Ishubhu leGlucose
Ishubhu le-glucose lisetshenziswa ekuqoqweni kwegazi ukuhlola okufana noshukela egazini, ukubekezelela ushukela, i-erythrocyte electrophoresis, i-anti-alkali hemoglobin ne-lactate.I-Sodium Fluoride eyengeziwe ivimbela ngempumelelo i-metabolism kashukela egazini futhi i-Sodium Heparin ixazulula ngempumelelo i-hemolysis.
Ngakho-ke, isimo sokuqala segazi sizohlala isikhathi eside futhi siqinisekise idatha ezinzile yokuhlola ushukela wegazi phakathi kwamahora angu-72.Isengezo ongasikhetha yi-Sodium Fluoride+Sodium Heparin, i-Sodium Fluoride+ EDTA.K2, i-Sodium Fluoride+EDTA.Na2.
Isivinini se-Centrifugation: 3500-4000 r / m
Isikhathi se-Centrifugation: 5min
Izinga lokushisa elinconyiwe lesitoreji: 4-25 ºC
7.Heparin Tube
Ishubhu le-Heparin lisetshenziswa ekuqoqweni kwegazi ukuze kuhlolwe i-plasma yomtholampilo, i-biochemistry yezimo eziphuthumayo kanye ne-rheology yegazi, njll. Ngokuphazanyiswa okuncane ekwakhiweni kwegazi futhi kungekho thonya kusayizi we-erythrocyte, ngeke kubangele i-hemolysis.Ngaphandle kwalokho, inezici zokuhlukaniswa okusheshayo kwe-plasma kanye nobubanzi obubanzi bokushisa okusebenzayo kanye nokuhambisana okuphezulu ne-serum index.
I-anticoagulant heparin yenza i-fibrinolysin isebenze, kuyilapho ivimbela i-thromboplastin, bese ifinyelela ibhalansi eguquguqukayo phakathi kwe-fibrinogen ne-fibrin, ngaphandle kwentambo ye-fibrin ohlelweni lokuhlola.Iningi lezinkomba ze-plasma lingaphinda phakathi kwamahora angu-6.
I-Lithium heparin ayinazo izici ze-sodium heparin kuphela, kodwa futhi ingasetshenziswa ekuhlolweni kwama-microelements ngaphandle komthelela we-sodium ion.Ukuze kuhlangatshezwane nezidingo ezihlukahlukene zelabhorethri yomtholampilo, i-KANGJIAN ingangeza ijeli yokuhlukanisa i-plasma ukuze yenze i-plasma yekhwalithi ephezulu.
Isivinini se-Centrifugation: 3500-4000 r / m
Isikhathi se-Centrifugation: 3min
Izinga lokushisa elinconyiwe lesitoreji: 4-25ºC
8.PT Ithubhu
Ishubhu ye-PT isetshenziselwa ukuhlolwa kokuhlangana kwegazi futhi isebenza ohlelweni lwe-fibrinolytic (PT, TT, APTT kanye ne-fibrinogen, njll.
Isilinganiso sokuxuba siyingxenye engu-1 ye-citrate kuya ezingxenyeni eziyi-9 zegazi.Isilinganiso esinembile singaqinisekisa ukusebenza komphumela wokuhlola futhi sigweme ukuxilonga kabi.
Njengoba i-sodium citrate inobuthi obuncane kakhulu, iphinde isetshenziselwe ukugcinwa kwegazi.Thatha umthamo wegazi owanele ukuze uqinisekise umphumela wokuhlola onembile.Ishubhu le-PT eline-double-deck linendawo encane efile, Engasetshenziswa ukuqapha ukuhlolwa kwe-v WF, F, imisebenzi yeplatelet, ukwelashwa kwe-Heparin.
Umbukiso Womkhiqizo
